YY1 antibody (pAb)

Catalog No: 61779 Format: 100 µl $445 Buy
Catalog No: 61780 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
63 kDa


ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

Application Notes

Validated Applications:
ChIP-Seq: 10 µl per ChIP
IP: 0.5 - 4 µl per IP
WB*: 1:1,000 - 1:5,000 dilution
IHC (FFPE): 1:1,000 dilution

*For optimal results, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. Visit activemotif.com to download the protocol.


This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


YY1 antibody (pAb) tested by ChIP-Seq.

YY1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from HeLa cells and 10 µl of YY1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 11.6 million sequence tags were mapped to identify YY1 binding sites.

YY1 antibody (pAb) tested by immunoprecipitation.

YY1 antibody (pAb) tested by Immunoprecipitation.
2 µl of YY1 antibody was used to immunoprecipitate YY1 from 250 µg of Raji nuclear cell extract (lane 2). 2 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the YY1 antibody at a dilution of 1:1,000.

YY1 antibody (pAb) tested by Western blot.

YY1 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of Raji cells probed with YY1 antibody at a 1:2,000 dilution.

YY1 antibody (pAb) tested by immunohistochemistry.

YY1 antibody (pAb) tested by Immunohistochemistry
Nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human adenocarcinoma. Top Panel: YY1 antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone)


YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with YY1 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting