Recombinant PTPN1 protein

Catalog No: 81034 Format: 50 µg $405 Buy
Catalog No: 81734 Format: 1 mg $2,600 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant PTPN1 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

PTPN1 (protein tyrosine phosphatase, non-receptor type 1, also known as PTP1B) is a member of the protein tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family have a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation as signaling molecules. Epidermal growth factor receptor (EGFR) and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. PTPN1 acts as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. PTPN1 dephosphorylates epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases.

Application Notes

Recombinant PTPN1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 1 mM pNPP was incubated with different concentrations of PTPN1 protein in 100 µl reaction system including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.

Protein Details

Recombinant PTPN1 protein was expressed in E. coli cells as the full length protein (accession number NP_002818.1) with an N-terminal GST tag. The molecular weight of the protein is 76.3 kDa.

 
Recombinant PTPN1 protein gel

Recombinant PTPN1 protein gel
10% SDS-PAGE gel stained with Coomassie blue.
MW: 76.3 kDa
Purity: > 45%

Recombinant PTPN1 protein activity assay

Recombinant PTPN1 protein activity assay
1 mM pNPP was incubated with different concentrations of PTPN1 protein in 100 µl reaction system including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.