Recombinant NgTet1 (1-321) protein
|Catalog No: 81148||Format: 100 µg||$420||Buy|
|Catalog No: 81848||Format: 1 mg||$2,650||Buy|
Recombinant NgTet1 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.
Ten Eleven Translocation (TET) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC). 5-hmC can be further oxidized into 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for iinitiating the enzymatic deamination process leading to cytosine demethylation.
The Tet family of dioxygenases is widely distributed across the tree of life, including in the heterolobosean amoeboflagellate Naegleria gruberi . NgTet1 (Naegleria Tet-like protein) is a homologue of mammalian Tet proteins. Like mammalian Tet proteins, NgTet1 acts on 5-mC and generates 5-hmC, 5-fC and 5-caC.
Recombinant NgTet1 protein is suitable for use in cytosine oxygenase assays, 5-mC sequencing, enzyme kinetics, inhibitor screening, and selectivity profiling.
Recombinant NgTet1 protein was expressed in E. coli cells as the full length protein (accession number XP_002667965.1) with an N-terminal 6xHis Tag. The molecular weight of NgTet1 is 38.7 kDa.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.