Acetyl-Lysine antibody (rAb)

Clone: RM101
Catalog No: 91315 Format: 100 µg $445 Buy

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Antibody Type:
Recombinant
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Reactivity:
Human, Not Species Specific

Applications

Western Blot Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml
ICC: 2 - 10 µg/ml

Immunogen

Acetyllysine-BSA

Buffer

PBS containing 50% Glycerol, 1% BSA, and 0.09% sodium azide.

 

Western blot of Acetyl-Lysine antibody (rAb).
HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 µg/ml.

Immunofluorescence stain of Acetyl-Lysine antibody (rAb).
HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 µg/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).

Background

Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histones can occur at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as HAT1 or GCN5. Acetylation of histones is often associated with transcriptional activation.

Clone RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting