PIXUL Multi-Sample Sonicator Overview
What Makes PIXUL Different
Unsurpassed Sonication Consistency at an Affordable Operating Cost
What our customers are saying about the PIXUL Multi-Sample Sonicator:
"When I used the PIXUL Multi-Sample Sonicator at The London BioFoundry, I found that it was very easy to use and maintain. I have confidence in PIXUL’s ability to perform reproducible sonication and I envision the successful application of the PIXUL system towards our ongoing synthetic biology applications."
"I used the PIXUL Multi-Sample Sonicator in my experiments and I was impressed by its ease of use, efficacy, and reproducibility."
PIXUL Multi-Sample Sonicator Data
Extremely Consistent Shearing of Genomic DNA
Highly consistent shearing of genomic DNA to an appropriate size for conversion into functional NGS libraries is integral to high quality and comparable next-generation sequencing data. Manual shearing with a probe sonicator or shearing with an inconsistent high-throughput sonicator can lead to sample-to-sample variability.
To test the consistency of the PIXUL Multi-Sample Sonicator, purified genomic DNA was loaded into all 96 wells of the PIXUL 96-well plate, processed, and fragmentation profiles examined for sonication consistency and efficiency. Highly consistent average fragment lengths and standard deviations were observed across the entire plate.
For consistent gDNA shearing, we recommend the PIXUL gDNA Shearing Kit.
PIXUL Delivers More Consistent Chromatin Shearing than Competitor C
ChIP-Seq studies benefit from relatively narrow and consistent chromatin shearing profiles, as this can improve the resolution of transcription factor binding sites or the locations of histone post-translational modifications.
To evaluate chromatin sonication consistency between different multi-sample sonicators, we compared chromatin shearing performed with the PIXUL to a leading Competitor C sonication system. PIXUL demonstrated more consistency in chromatin shearing than the Competitor C system.
PIXUL Enables More Consistent Sonication and Higher Chromatin Recovery than Competitor D
ChIP-Seq studies have historically required chromatin preparation from millions of cells because the limitations created by inefficienct sonication. As ChIP-Seq techniques have progressed, the number of input cells required has decreased by maximizing the efficiency of sonication and chromatin recovery.
PIXUL 96-well plates are compatible with cell culture, fixation, and sonication steps there's no need to transfer samples from culture plates to sonication plates and risk sample loss. Compared to Competitor D, PIXUL exhibits a tighter size distribution of chromatin fragments following sonication, as well as higher chromatin recovery (data not shown).
PIXUL Delivers Equivalent Exome Sequencing Results as Competitor C for a Fraction of the Cost
PIXUL was compared to Competitor C in an exome sequencing experiment, which requires DNA shearing as a first step. Using 500 ng of input DNA, samples were sheared with either PIXUL or Competitor C, libraries were constructed, enriched for the exome, and sequenced. Several sequencing metrics were analyzed to determine the PIXUL instrument produced exome libraries that performed equivalently to Competitor C, at just a fraction of the sample preparation cost for the instrument and consumables.
PIXUL Multi-Sample Sonicator Storage & Contents
The PIXUL Multi-Sample Sonicator is shipped at room temperature and should be operated in an environment of 15°C - 25°C and maximum relative humidity of 85%.
Each unit includes the following components:
- PIXUL instrument
- Power cord
- Drain hose
PIXUL Multi-Sample Sonicator Publications
Search our database of customer publications that have used our PIXUL Multi-Sample Sonicator.
PIXUL Multi-Sample Sonicator Documents
- PIXUL™ Multi-Sample Sonicator Manual (After Nov 2021)
- PIXUL™ Multi-Sample Sonicator Manual (Through Oct 2021)
TechNotes & Supplemental Protocols
- Supplemental Protocol: Yeast Lysis with PIXUL™ Multi-Sample Sonicator
- Supplemental Protocol: Bacterial Lysis with PIXUL™ Multi-Sample Sonicator
- TechNote: Sample Preparation for Proteomics Applications with PIXUL™
- TechNote: Sonicating Limited Tissue Samples with PIXUL™ Multi-Sample Sonicator for ChIP-Seq Analysis
- TechNote: Sonication Recommendations for ChIP
- Quick Guide: PIXUL™ Multi-Sample Sonicator
- Quick Guide: Shearing Chromatin from Frozen Tissues with PIXUL™
You might also be interested in:
PIXUL™ is sold under an exclusive license to patents owned by Matchstick Technologies Inc. and University of Washington, specifically Matula, T.J.; Bomsztyk, K.; Darlington, G.P., Maxwell, A.D.; MacConaghy, B.E., Reed, J. “Ultrasound system for shearing cellular material”. US Patent US10809166. European Patent EP3169451.
|PIXUL™ Multi-Sample Sonicator||1 unit||53130||Get Quote|
|PIXUL™ Coupling Fluid||1 L||53136||$235||Buy|
|PIXUL™ 96-well Plate with Sealer||1 unit||53139||$30||Buy|