Because no single control can be ideal for all experimental conditions, Active Motif offers a panel of positive and negative control constructs for the LightSwitch™ 3´UTR Reporter Vector. These controls are used to measure background, to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. So, instead of one control construct being co-transfected with every experimental plasmid, different control vectors can be used in your reporter assay experiment, each in its own well, in addition to the constructs being tested. Using the most suitable positive and negative control vectors for your specific experiment will give you the highest degree of confidence in your results.
Information on all LightSwitch 3´UTR Control Vectors is shown under the 3´UTR Controls tab below.
IMPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
Name | Type of Control | Product Info | Format | Cat No. | Price | |
---|---|---|---|---|---|---|
LightSwitch™ ACTB 3´UTR Control | Positive control | Get Info | 5 µg | 32013 | $250 | Add to Cart |
LightSwitch™ GAPDH 3´UTR Control | Positive control | Get Info | 5 µg | 32014 | $250 | Add to Cart |
LightSwitch™ LDHA 3´UTR Control | Positive control | Get Info | 5 µg | 32015 | $250 | Add to Cart |
LightSwitch™ PPIA 3´UTR Control | Positive control | Get Info | 5 µg | 32016 | $250 | Add to Cart |
LightSwitch™ Random 3´UTR Control 1 | Negative control | Get Info | 5 µg | 32017 | $250 | Add to Cart |
LightSwitch™ Random 3´UTR Control 2 | Negative control | Get Info | 5 µg | 32018 | $250 | Add to Cart |
LightSwitch™ Random 3´UTR Control 3 | Negative control | Get Info | 5 µg | 32019 | $250 | Add to Cart |
LightSwitch™ Random 3´UTR Control 4 | Negative control | Get Info | 5 µg | 32020 | $250 | Add to Cart |
LightSwitch™ 3´UTR Reporter Vector | Empty vector | Vector Info | 5 µg | 32022 | $250 | Add to Cart |
LightSwitch™ Luciferase Assay System Profile |
LightSwitch™ miR-122 White Paper |
LightSwitch™ miRNA Reference Guide |
Tools for Disease Research |
LightSwitch™ 3´UTR GoClone® Data Sheet |
Empty 3´UTR vector: The empty 3´UTR vector contains the RenSP luciferase reporter gene and its constitutive promoter. This construct may serve as a positive control for the transfection because of its promoter. It may also be utilized for creating additional constructs. For a vector map, annotations and primer & restriction sites, please see the Empty LightSwitch™ Reporter Vectors page.
Housekeeping gene 3´UTR vectors: Housekeeping control constructs contain the 3´UTRs for common housekeeping genes cloned downstream of the RenSP luciferase reporter gene.
Random control 3´UTR vectors: Random control constructs contain non-conserved, non-genic, and non-repetitive human genomic fragments.
3´UTRs of housekeeping genes
Catalog No. | Name | Description | Coordinates | Sequence |
---|---|---|---|---|
32013 | LightSwitch™ ACTB 3´UTR Control |
Beta-actin | chr7–: 5340002 - 5340681 |
Get Info |
32014 | LightSwitch™ GAPDH 3´UTR Control |
Glyceraldehyde-3-phosphate dehydrogenase |
chr12+: 6517534 - 6517833 |
Get Info |
32015 | LightSwitch™ LDHA 3´UTR Control |
Lactose dehydrogenase A | chr11+: 18385382 - 18386064 |
Get Info |
32016 | LightSwitch™ PPIA 3´UTR Control |
Peptidylprolyl isomerase A | chr7+: 44614226 - 44616012 |
Get Info |
Random genomic fragments in 3´UTR vector
Catalog No. | Name | Coordinates | Sequence |
---|---|---|---|
32017 | LightSwitch™ Random 3´UTR Control 1 |
chr13+: 82190847 - 82193385 |
Get Info |
32018 | LightSwitch™ Random 3´UTR Control 2 |
chr12+: 82120892 - 82121673 |
Get Info |
32019 | LightSwitch™ Random 3´UTR Control 3 |
chr13+: 26144830 - 26145608 |
Get Info |
32020 | LightSwitch™ Random 3´UTR Control 4 |
chr22+: 1645930 - 1646850 |
Get Info |