Cart
Products
The LightSwitch™ Transfection Optimization Kit includes the key reagents and protocols needed to optimize transfection conditions for your cell line or condition of interest. The kit utilizes the FuGENE® HD Transfection Reagent, which is one of the most robust transfection reagents available, together with our LightSwitch™ Luciferase Assay Kit and LightSwitch Positive & Negative Control GoClone® constructs.
The kit was designed to help you determine what our extensive experience with transfection and reporter assays has identified as the 3 key variables to successfully transfect a variety of different cell lines:
- FuGENE to plasmid ratio
- Cells seeded per well
- Duration of transfection
Use of the LightSwitch Transfection Optimization Kit will help you consistently achieve higher transfection efficiencies, which will greatly improve the results of your luciferase reporter assay experiments.
The LightSwtich Transfection Optimization Kit is no longer available from Active Motif. However, the components can still be purchased separately. Please see below:
- LightSwitch™ Positive Control GoClone® construct Active Motif Cat No. 32006, RPL10 Promoter Control
- LightSwitch™ Negative Control GoClone® construct Active Motif Cat No. 32021, Empty Promoter Reporter Vector
- FuGENE® HD Transfection Reagent Available from FuGene.
- LightSwitch™ Luciferase Assay Kit (100 assay size)
– Lyophilized 100X LightSwitch™ Assay Substrate
– LightSwitch™ Substrate Solvent
– LightSwitch™ Assay Buffer
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| LightSwitch™ Transfection Optimization Kit | 1 kit | 32041 | Discontinued | |
| LightSwitch™ Transfection Optimization Kit Manual |
| LightSwitch™ Luciferase Assay System Profile |
| Gene Regulation Products & Services Brochure |
| Tools for Disease Research |
Contents & Storage
The LightSwitch™ Transfection Optimization Kit contains:
- LightSwitch™ Positive Control GoClone® construct (-20°C)
- LightSwitch™ Negative Control GoClone® construct (-20°C)
- FuGENE® HD Transfection Reagent (4°C)
- LightSwitch™ Luciferase Assay Kit (100 assay size)
– Lyophilized 100X LightSwitch™ Assay Substrate (-20°C)
– LightSwitch™ Substrate Solvent (room temperature)
– LightSwitch™ Assay Buffer (-20°C) - Protocol
All reagents are guaranteed stable for 6 months when stored properly.
