Recombinant PBRM1 (613-734) protein

Aliases: BRG1-associated factor 180 (BAF180), Polybromo 1, PB1; BAF180
Catalog No: 31385 Format: 100 µg $405 Buy
Catalog No: 31785 Format: 1 mg $3,500 Buy
Expressed In: E. coli Protein Species: Human

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Recombinant PBRM1 (613-734) protein expressed in E. coli and supplied in 25 mM Tris-HCl pH 8.0, 500 mM NaCl, 0.04% Triton X-100, 20% glycerol, 1 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.


Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers.

Application Notes

Recombinant PBRM1 (613-734) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Protein Details

The peptide corresponding to amino acids 613 - 734 that contains the bromodomain sequences of PBRM1 (accession number NP_060635.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 20.513 kDa. It shows binding specificity for acetylated H3K14.

PBRM1 (613-734) Coomassie gel

Recombinant PBRM1 (613-734) protein gel.
PBRM1 (613-734) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

PBRM1 (613-734) HTRF activity assay

Recombinant PBRM1 (613-734) HTRF activity assay.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with PBRM1 (613-734) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0 and 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.