Recombinant KMT2A (MLL1)-SET protein

Catalog No: 31419 Format: 50 µg $430 Buy
Catalog No: 31819 Format: 1 mg $2,415 Buy

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Expressed In: E. coli Protein Species: Human


A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.


Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), or MLL, also known as KMT2A or HRX, is a Trithorax-group protein that function collectively to promote gene expression. MLL is a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The protein contains multiple conserved functional domains. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

The peptide corresponding to amino acids 3735 - 3973 of the KMT2A (MLL1) protein (accession number NP_001184033.1) was expressed in E. coli. The peptide contains the SET domain of MLL that is responsible for methyltransferase activity. Recombinant MLL / HRX - SET contains an N-terminal GST-Tag with a molecular weight of 53.7 kDa. The recombinant protein is >92% pure by SDS-PAGE.


Recombinant KMT2A (MLL1)-SET protein gel.
MLL / HRX - SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant KMT2A (MLL1)-SET binding assay.
KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4°C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.