Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

MLL / HRX antibody (mAb)

Clone: N4.4
Catalog No: 39829 Format: 100 µg $425 Buy Now
Catalog No: 39830 Format: 10 µg $99 Buy Now

Request a quote for a bulk orderBulk Order Button

Antibody Type:Monoclonal
Purification:Protein G Chromatography
Molecular Weight:440 kDa
Reactivity:Human, Mouse


ChIP Validated Western Blot Validated Immunoprecipitation Validated

for MLL / HRX antibody (mAb) (Clone N4.4)Application Notes

Published Applications:
See references for more information. Individual optimization may be required.

for MLL / HRX antibody (mAb) (Clone N4.4)Immunogen

This MLL / HRX antibody was raised against a recombinant protein corresponding to amino acids 161-356 of mouse MLL.

for MLL / HRX antibody (mAb) (Clone N4.4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MLL / HRX antibody (mAb) (Clone N4.4)References

for MLL / HRX antibody (mAb) (Clone N4.4)Background

MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a SET-domain containing histone methyltransferase specific for lysine 4 of histone H3. It serves as a transcriptional activator for many target genes, including several HOX genes. MLL is a frequent target for translocations in acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL) and mixed lineage leukemia (MLL). A key feature of MLL fusion proteins is their ability to efficiently transform hematopoietic cells into leukemia stem cells. Leukemogenic MLL translocations encode MLL fusion proteins that have lost H3 Lys4 methyltransferase activity. MLL is found in a complex of proteins that includes ASH2L, WDR5, DPY30 and RBBP5.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MLL / HRX antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MLL / HRX antibody (mAb).

for MLL / HRX antibody (mAb) (Clone N4.4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MLL / HRX antibody (mAb) (Clone N4.4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

MLL / HRX antibody (mAb)