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Recombinant USP7 protein
| Aliases: Ubiquitin Specific Peptidase 7, Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) |
| Catalog No: 31525 | Format: 20 µg | $450 | Add to Cart |
| Catalog No: 31925 | Format: 1 mg | $4,050 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. USP7 de-ubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, UHRF1, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. USP7 de-ubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. USP7 also de-ubiquitinates FOXO4 and inhibits FOXO4-induced transcriptional activity. Together with DAXX, USP7 is involved in the de-ubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 de-ubiquitinates UHRF1, which prevents its degradation and promoting DNA methylation by DNMT1.
Application Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Protein Details
Recombinant human USP7 was expressed in a baculovirus expression system as the full length protein (accession number NP_003461.2) with an N-terminal 6xHis Tag. The molecular weight of the protein is 131.3 kDa. The purity of USP7 protein is >80% by SDS-PAGE.
References
Human
Recombinant USP7 protein gel
8% SDS-PAGE gel stained with Coomassie Blue
MW: 131.3 kDa
Purity: >80%
DUB-Detector assay (Cat. No. 40110) for USP7 protein activity
100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.
DUB-Detector assay (Cat. No. 40110) for USP7 protein activity
100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
