Ubiquitin antibody (mAb)

RRID: AB_2793327
Clone: P4D1
Catalog No: 39741 Format: 100 µg $460 Buy
Catalog No: 39742 Format: 10 µg $115 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB

For additional information, please see the original publication for the generation of this clone: Kahana, A., and Gottschling, D.E., Mol. Cell. Biol. 19: 6608-6620, 1999.

This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91257.

Immunogen

This Ubiquitin antibody was raised against purified bovine ubiquitin.

Buffer

PBS, 30% glycerol and 0.035% sodium azide.

 

Ubiquitin antibody specifity
Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with Ubiquitin antibody at 2 µg/ml.

Background

Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting