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2 x 100 µg of HeLa nuclear extract at 2.5 µg/µl.
Background
HeLa nuclear extract was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.
Application Notes
HeLa nuclear extract is recommended for use in various applications across many research areas, including cancer, virology, and gene mapping studies.
Extract Origin
Human Cervix Adenocarcinoma
Extract Composition
HeLa nuclear extract is supplied in lysis buffer (20 mM Hepes (pH 7.5), 350 mM NaCl, 20% glycerol, 1% Igepal, 1 mM MgCl2, 0.1 mM EGTA, 5 mM DTT and protease inhibitors.
Quality Control
Extracts have been quality control tested by Western blot and TransAM® Transcription Factor ELISAs.
Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
