Contents
2 x 100 µg of HeLa nuclear extract at 2.5 µg/µl.
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Extract Origin
Human Cervix Adenocarcinoma
Extract Composition
The HeLa whole-cell extract has been collected in Whole-Cell Lysis Buffer. Whole-Cell Lysis Buffer consists of 25mM Tris-HCl pH 7.5, 150 mM NaCl, 5% glycerol, 1% Igepal-CA630, and 5 mM MgCl2. The protein content has been determined by a Bradford-based assay.
Quality Control
Extracts have been quality control tested by Western blot.
HeLa whole-cell extract (20 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution).
Storage
To ensure stability, extracts should be stored at -80ºC. We recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of arrival.