AML-1/Runx1 antibody (pAb)

RRID: AB_2793147
Aliases: CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1
Catalog No: 39000 Format: 100 µl Discontinued

Antibody Type:
Affinity Purified
Molecular Weight:
53 kDa
Human, Mouse


ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes

This product has been discontinued:
Validated Applications:
IHC (FFPE): 1:100 - 1:500
WB*: 1:500 - 1:1000

Published Applications:

See references for more information. Individual optimization may be required.

*For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.


This AML-1/Runx1 antibody was raised against a synthetic peptide corresponding to amino acid residues 231-245 of human AML-1/Runx1.


PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.


AML-1/Runx1 antibody tested by Western blot.
Detection of AML-1/Runx1 by Western blot analysis. Positive detection of AML-1/Runx1 in nuclear extracts derived from Jurkat cells at 1:1,000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions.

AML-1 / Runx1 antibody (pAb) tested by Immunohistochemistry
Nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human breast carcinoma. Top Panel: AML-1/Runx1 antibody at 1:100 dilution. Bottom Panel: No primary antibody (2nd step antibody alone)


AML-1 (acute myeloid leukemia 1, Runx1, CBFA2, PEBP2αB) is a transcription factor (activator) for various hematopoietic-specific genes (for example, GM-CSF, CSF1R and TCRb). AML-1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoters and enhancer elements. AML-1 is believed to be involved in the development of normal hematopoiesis and is commonly translocated in hematopoietic cancers.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AML-1/Runx1 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.


This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting