ETO / RUNX1T1 antibody (pAb)

Aliases: CDR, MTG8, AML1T1, ZMYND2, CBFA2T1
Catalog No: 61109 Format: 100 µl $435 Buy
Catalog No: 61110 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
68 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This ETO / RUNX1T1 antibody was raised against a peptide within the internal region of human ETO / RUNX1T1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
ETO / RUNX1T1 antibody (pAb) tested by immunoprecipitation.

ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation.
10 µl of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500.

ETO / RUNX1T1 antibody (pAb) tested by Western blot.

ETO / RUNX1T1 antibody (pAb) tested by Western Blot:
K-562 nuclear extract (20 µg per lane) probed with the ETO / RUNX1T1 antibody (pAb) at a dilution of 1:500.

Background

ETO / RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO / RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT / NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO / RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO / RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8;21) translocation which gives rise to the AML-ETO fusion protein.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for ETO / RUNX1T1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting