EGF Receptor phospho Tyr992 antibody (pAb)

Aliases: EGFR, ERBB, HER1, mENA, ERBB1, PIG61
Catalog No: 39094 Format: 200 µl $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
170 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This EGF Receptor antibody was raised against a synthetic phosphopeptide corresponding to the amino acid residues region that contains Tyr992 of human Phosphorylated-EGFR.

Buffer

12 mM sodium borate, 50 mM boric acid, 37 mM NaCl, 5 mM EDTA and 50% glycerol.

References

 
EGF Receptor phospho Tyr992 antibody (pAb) tested by Western blot.

EGF Receptor phospho Tyr992 pAb tested by Western blot.
Detection of Phospho-EGFR by Western blot. The analysis was performed using A-431 nuclear extract (EGF treated) and EGF Receptor phospho Tyr992 pAb at a 1:500 dilution. The reactions were incubated in the absence (lane 1) and presence of immunizing peptide (lane 2), and in the presence of non-phosphorylated peptide (lane 3), which confirms the specificity of the antibody for the phosphorylated form of the EGF receptor.

Background

EGFREpidermal Growth Factor Receptor is a member of the superfamily of transmembrane receptor tyrosine kinases and is widely expressed. Upon ligand binding, EGFR dimerizes, autophosphorylates and becomes active. Many signaling pathways are initiated through the EGFR and related ligands. Phosphorylation of Tyr 992 results in the binding and activation of Phospholipase-C gamma (PLC-gamma).

Positive Control

Active Motif's A-431 nuclear extract (EGF treated) (Catalog No. 36003) can be used as a positive control for EGF Receptor phospho Tyr992 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting