Recombinant EGFR protein
|Aliases: ERBB, HER1, mENA, ERBB1, PIG61, NISBD2|
|Catalog No: 31165||Format: 10 µg||$395||Buy|
Expressed In: Baculovirus Protein Species: Human
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Recombinant EGFR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~91.002 kDa. The activity of the protein is ~ 93 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 10 µg/50 µl, Recombinant EGFR: 40 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.