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PP2A antibody (pAb)

Aliases: PPP2R4, PR53, PTPA
Catalog No: 39192 Format: 200 µl $425 Buy Now
Catalog No: 39193 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:36 kDa
Reactivity:Human, Mouse, Other (Wide Range), Rat


ChIP Validated Western Blot Validated

for PP2A antibody (pAb)Application Notes

Validated Applications:
ChIP: 2 - 10 µl per ChIP
WB: 1:250 - 1:2,000 dilution

Published Applications: see references

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for PP2A antibody (pAb)Immunogen

This PP2A antibody was raised against a peptide corresponding to amino acids 288-303 of human PP2A.

for PP2A antibody (pAb)Buffer

Peptide affinity purified IgG containing 30% glycerol, 0.035% sodium azide and 1 mg/ml BSA. Sodium azide is highly toxic.

for PP2A antibody (pAb)References

PP2A antibody (pAb) tested by ChIP.

PP2A antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 µl of PP2A antibody or the equivalent amount of IgG as a negative control.

PP2A antibody (pAb) tested by Western blot.

PP2A pAb tested by Western blot.
Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).

for PP2A antibody (pAb)Background

PP2AProtein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers.

for PP2A antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PP2A antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PP2A antibody (pAb).

for PP2A antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PP2A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

PP2A antibody (pAb)

Data Thumbnails

PP2A antibody (pAb) tested by ChIP.

ChIP of PP2A pAb.
(Click image to enlarge and see details.)

PP2A antibody (pAb) tested by Western blot.

Western blot of PP2A pAb.
(Click image to enlarge and see details.)