SMC1 phospho Ser957 antibody (pAb)

Aliases: SMC1A, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, SMC1alpha
Catalog No: 39631 Format: 200 µl $435 Buy
Catalog No: 39632 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
160 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Immunogen

This SMC1 phospho Ser957 antibody was raised against a peptide containing phospho-serine 957 of human SMC1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
SMC1 phospho Ser957 antibody (pAb) tested by Western blot.

SMC1 phospho Ser957 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with Catalog No. 39631 (1:1,000 dilution). Lane 1: untreated cells. Lane 2: cells treated with etoposide (100 µM for 2hrs).

SMC1 phospho Ser957 antibody (pAb) tested by dot blot analysis.

SMC1 phospho Ser957 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of SMC1 phospho Ser957 pAb for phospho Ser957 of SMC1. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 957. Lane 2: unmodified peptide containing serine 957.

Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems.

Positive Control

Nuclear extract of HeLa cells treated with etoposide (100 µM for 2 hours) can be used as positive control for SMC1 phospho Ser957 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting