SMC4 antibody (pAb)

Aliases: CAPC, CAP-C, SMC-4, SMC4L1, hCAP-C
Catalog No: 61129 Format: 100 µl $435 Buy
Catalog No: 61130 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
175 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This SMC4 antibody was raised against a peptide from the N-terminus of human SMC4.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
SMC4 antibody (pAb) tested by immunoprecipitation.

SMC4 antibody (pAb) tested by Immunoprecipitation.
10 µl of SMC4 antibody was used to immunoprecipitate SMC4 from 500 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC4 antibody at a dilution of 1:1,000.

SMC4 antibody (pAb) tested by Western blot.

Western blot of SMC4 antibody.
Nuclear extract of HeLa cells (20 μg) probed with SMC4 antibody (1:1,000).

Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC4 is the central component of the condensin complex, a complex required for conversion of interphase chromatin into condensed mitotic chromosomes. SMC4 forms a heterodimer with SMC2 and contains a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin. During mitosis, most of the condensin complex is associated with the chromatin.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMC4 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting