CREB antibody (mAb)

Clone: 82B514
Aliases: CREB1
Catalog No: 40961 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
43 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This CREB antibody was raised against a synthetic peptide corresponding to human CREB.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
CREB antibody (mAb) tested by Western blot.

CREB mAb tested by Western blot.
Detection of CREB by Western blot analysis. A protein band of approximate molecular weight of 43 kDa was detected using HeLa whole-cell extract and CREB mAb at a dilution of 2 µg/ml.

Background

CREB (cAMP response element binding) transcription factor binds as a homodimer to specific DNA sequences called cAMP response elements (CRE) and regulates transcription. CREB belongs to the leucine zipper family of DNA binding proteins. CREB induces transcription in response to hormonal stimulation.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for CREB antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting