PRMT5 antibody (pAb)

Aliases: JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
Catalog No: 61001 Format: 100 µl $435 Buy
Catalog No: 61002 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
75 kDa


ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.


This PRMT5 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human PRMT5.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


Strahan, R.C., et. al. 2017. "KSHV encoded ORF59 modulates histone arginine methylation of the viral genome to promote viral reactivation." PLoS Pathog. Jul 5;13(7):e1006482. (ChIP-Seq)

PRMT5 antibody (pAb) tested by Western blot.

Western blot of PRMT5 antibody.
Cytoplasmic extract of Jurkat cells (30 µg per lane) probed with PRMT5 antibody (1:1,000).


PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates PIWI proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.

Positive Control

Active Motif's Jurkat cytoplasmic extract (Catalog No. 36402) can be used as a positive control for PRMT5 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PRMT5 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting