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Cell, Acid & Tissue Extracts

Jurkat cytoplasmic extract

Catalog No: 36402 Format: 200 µg $170 Buy Now

for Jurkat cytoplasmic extractContents

200 µg of Jurkat cytoplasmic extract at 5 µg/µl.

for Jurkat cytoplasmic extractBackground

Jurkat cytoplasmic extract was prepared from cell cultures of the Jurkat human CD4(+) T cell lymphoblast-like cell line. The Jurkat cell line was established from the propagation of peripheral blood cells of a 14 year old boy with T cell leukemia. These cells are primarily used in research as a T cell model system, particularly in studies of T cell signaling, T cell leukemia, and response to viral infection. Jurkat cells are frequently used in HIV-related research to examine the mechanisms governing T cell-mediated immunity in response to HIV infection. Stimulation of these cells with phorbol esters and either lectins or monoclonal antibodies leads to activation of T cell antigen receptors and induction of IL-2 expression. Because these cells can produce IL-2 in response to stimulation, they are frequently used in research to study the regulation of cytokine expression and differentiation. However, the primary use of Jurkat cells is to study the response of various cancers to radiation or drug treatments.

for Jurkat cytoplasmic extractApplication Notes

Jurkat cytoplasmic extract is specifically recommended for use in studies related to 1) T cell-mediated immunity and response to viral infection (e.g. HIV), 2) cytokine induction and cell differentiation, and 3) cellular response to cancer therapies.

for Jurkat cytoplasmic extractExtract Origin

Human T lymphoblast; acute T cell leukemia

for Jurkat cytoplasmic extractExtract Composition

Jurkat cytoplasmic extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for Jurkat cytoplasmic extractQuality Control

Extracts have been quality control tested by Western blot.

for Jurkat cytoplasmic extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Jurkat cytoplasmic extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Jurkat cytoplasmic extract