NR2F2 / COUP2 antibody (pAb)

RRID: AB_2614942
Aliases: ARP1, NF-E3, NR2F1, SVP40, COUPTFB, TFCOUP2, COUPTFII
Catalog No: 61213 Format: 100 µl
Catalog No: 61214 Format: 10 µl

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

This product has been discontinued

Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq: 5 µl each
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP

For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.05% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This NR2F2 / COUP2 antibody was raised against a peptide within the N-terminal region of human NR2F2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

NR2F2 / COUP2 (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human endometrial stromal cells and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 28 million sequence tags were mapped to identify NR2F2 binding sites. The purple track shows NR2F2 binding across a 1.2 million bp region on chromosome 12. Binding sites showed good correlation with the ENCODE active regulatory elements track (yellow).

NR2F2 / COUP2 (pAb) tested by Western blot.
Detection of NR2F2 / COUP2 by Western blot. The analysis was performed using 20 µg MCF-7 nuclear extract and NR2F2 / COUP2 (pAb) at a 1:500 dilution.

Background

NR2F2 / COUP2 (ARP1, TFCOUP2, COT2, COUP-TF2) is a member of the nuclear receptor superfamily related to the COUP transcription factor. It interacts with several nuclear receptor co-activator proteins to activate transcription. NR2F2 / COUP2 plays important roles in signaling pathways in multiple endocrine systems and is crucial for differentiation of the trophoblast during development.

Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for NR2F2 / COUP2 antibody.

qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NR2F2 / COUP2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting