MCF-7 nuclear extract

Catalog No: 36017 Format: 200 µg $175 Buy

Contents

2 x 100 µg of MCF-7 nuclear extract at 2.5 µg/µl.

Background

MCF-7 nuclear extract was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer.

Application Notes

MCF-7 nuclear extract is specifically recommended for studies related to breast cancer research.

Extract Origin

Human breast carcinoma

Extract Composition

MCF-7 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and TransAM® Transcription Factor ELISAs.

 

Figure 1: Monitoring ERα activation with the TransAM ER Kit.
Different amounts of untreated and H2O2 post-treated nuclear extracts from MCF-7 cells are tested for ER activity using the TransAM ER Kit.

Figure 1: Monitoring Sp1 and Sp3 activity with the TransAM Sp1/Sp3 Kit.
Different amounts of untreated and H2O2-post treated nuclear extracts from MCF-7 cells are tested for Sp1 and Sp3 activity using the TransAM Sp1/Sp3 Kit.

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.