MOF / MYST1 antibody (pAb)

RRID: AB_2793566
Aliases: KAT8, hMOF, ZC2HC8
Catalog No: 61245 Format: 100 µl $460 Buy
Catalog No: 61246 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
65 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This MOF / MYST1 antibody was raised against a peptide within the N-terminal region of human MOF / MYST1 (MYST histone acetyltransferase 1).

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

MOF / MYST1 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with MOF / MYST1 antibody at a 1:2,000 dilution.

MOF / MYST1 antibody (pAb) tested by Western blot.
Whole-cell extract of HeLa cells (30 µg) probed with MOF / MYST1 pAb at a dilution of 1:500.

Background

MOF / MYST1 (ortholog of Drosophila males absent on the first (MOF), MYST histone acetyltransferase 1, Histone acetyltransferase KAT8) is a histone acetyltransferase which may be involved in transcriptional activation. Member of the MYST family of histone acetyltransferases. Contains a chromodomain and C2HC-type zinc finger motif. Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Interacts with ATM through the chromodomain and may influence the function of ATM.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MOF / MYST1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MOF / MYST1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting