MOF / MYST1 antibody (pAb)

Aliases: KAT8, hMOF, ZC2HC8
Catalog No: 61245 Format: 100 µl $435 Buy
Catalog No: 61246 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
65 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution

Immunogen

This MOF / MYST1 antibody was raised against a peptide within the N-terminal region of human MOF / MYST1 (MYST histone acetyltransferase 1).

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
MOF / MYST1 antibody (pAb) tested by immunofluorescence.

MOF / MYST1 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with MOF / MYST1 antibody at a 1:2,000 dilution.

MOF / MYST1 antibody (pAb) tested by Western blot.

MOF / MYST1 antibody (pAb) tested by Western blot.
Whole-cell extract of HeLa cells (30 µg) probed with MOF / MYST1 pAb at a dilution of 1:500.

Background

MOF / MYST1 (ortholog of Drosophila males absent on the first (MOF), MYST histone acetyltransferase 1, Histone acetyltransferase KAT8) is a histone acetyltransferase which may be involved in transcriptional activation. Member of the MYST family of histone acetyltransferases. Contains a chromodomain and C2HC-type zinc finger motif. Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Interacts with ATM through the chromodomain and may influence the function of ATM.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MOF / MYST1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MOF / MYST1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting