OGA/O-GlcNAcase antibody (pAb)

Aliases: MGEA5, OGA, MEA5, NCOAT
Catalog No: 61425 Format: 100 µl $435 Buy
Catalog No: 61426 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
115 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This antibody was raised against a peptide within the N-terminal region of human OGA/ O-GlcNAcase.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
OGA/O-GlcNAcase antibody (pAb) tested by immunoprecipitation.

OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation.
10 µl of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.

OGA/O-GlcNAcase antibody (pAb) tested by Western blot.

OGA/O-GlcNAcase pAb tested by Western blot.
OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 µg HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.

Background

OGA / O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for OGA/O-GlcNAcase antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting