Recombinant PARP1 protein

Catalog No: 81037 Format: 20 µg $405 Buy
Catalog No: 81737 Format: 1 mg $3,500 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant PARP1 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

PARP1 (Poly(ADP-Ribose) Polymerase 1, also known as PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1) is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. Poly(ADP-ribosyl)ation is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage

For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant PARP1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005362.3) with an N-terminal FLAG tag. The molecular weight of PARP1 is 115.1 kDa.

 
Recombinant PARP1 protein gel

Recombinant PARP1 protein gel
8% SDS-PAGE gel stained with Coomassie blue.
MW: 115.1 kDa
Purity: ≥8

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.