PARP-1 N-terminal antibody (pAb)

RRID: AB_2793257
Aliases: PARP, PPOL, ADPRT, ARTD1, ADPRT1, ADPRT 1, pADPRT-1
Catalog No: 39559 Format: 100 µl $470 Buy
Catalog No: 39560 Format: 10 µl $115 Buy
Catalog No: 39061 Format: 50 µl $275 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
120 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 8 µl per ChIP
WB: 1:5,000 - 1:50,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR, ChIP-Seq, ChIP-chip
IHC/IF
WB
DB
Proximity Ligation Assay (PLA)

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This PARP-1 N-terminal antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1 was used to generate this PARP-1 antibody.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61639) of this antibody that was purified by Protein A Chromatography is also available.

 

PARP-1 N-terminal antibody tested by ChIP analysis.
Chromatin IP performed with chromatin from 3T3 cells using 8 µl of PARP-1 N-terminal antibody or rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PPARG2 gene. Data are presented as percent of input from cells with a PARP-1 knock-down (black bars) or a control knock-down (gray bars).

PARP-1 N-terminal antibody tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with PARP-1 N-terminal antibody at a dilution of 1:20,000.

Background

PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.

For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PARP-1 N-terminal antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-1 N-terminal antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting