AbFlex® BrdU antibody (rAb)

RRID: AB_2793827
Catalog No: 91269 Format: 100 µg $350 Buy
Catalog No: 91270 Format: 10 µg $75 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
N/A
Reactivity:
Human, Not Species Specific

Applications

Immunofluorescence Validated

Application Notes

Applications Validated by Active Motif:
IF: 0.5 - 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61273.

Immunogen

This antibody was raised against Bromodeoxyuridine conjugated to BSA (Bovine Serum Albumin).

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 

AbFlex® BrdU antibody (rAb) tested by Immunofluorescence
HeLa cells were stained with 0.5 µg/ml BrdU antibody, followed by anti-mouse IgG2a – Alexa Fluor 488 (lower panel) or DAPI (upper panel). Prior to staining, HeLa cells were treated for 1 hr at 37°C in cell culture medium supplemented with 10 µM BrdU. DNA was denatured wtih NaOH treatment for detection of BrdU incorporation in cells undergoing DNA replication at the time of labeling.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® BrdU antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

BrdU(Bromodeoxyuridine) is a thymidine analogue, capable of being incorporated into DNA in place of thymidine during DNA synthesis. By pulse labeling with BrdU, the DNA synthesis in the cells can be analyzed by detection with anti-BrdU antibodies. Antibodies against BrdU recognize BrdU in single-stranded DNA (produced by partial denaturation of double-stranded DNA), as well as free BrdU or BrdU coupled to a protein carrier.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting