LexA DNA-binding Domain antibody (pAb)

Catalog No: 39184 Format: 100 µl $435 Buy
Catalog No: 39185 Format: 10 µl $105 Buy

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Antibody Type:
Other (Not Species Specific)


ChIP Validated Western Blot Validated

Application Notes

Validated Applications:
IP: 1 µl/ 200 µg lysate
WB: 1:25,000 - 1:100,000 dilution

Published Applications:

Validation for Western blot was confirmed using a LexA-Rb fusion and a LexA-PP2A, β subunit fusion expressed in budding yeast. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This LexA DNA-binding Domain antibody was raised against recombinant protein corresponding to full-length E. coli LexA.


Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


LexA DNA-binding Domain antibody (pAb) tested by Western blot.

LexA DNA-binding Domain pAb tested by Western blot.
20 µg of crude yeast protein extracts probed with LexA DNA-binding Domain pAb (1:100,000 dilution).
    Lane 1: Extract from wild-type yeast.
    Lane 2: Extract from yeast expressing a LexA-Retinoblastoma (Rb) fusion protein.


LexA is a prokaryotic protein that normally functions to repress the expression of DNA-damage response genes. The LexA DNA binding domain is frequently used as an epitope tag or fused to the "bait" protein as part of the yeast two-hybrid protein interaction system.

Positive Control

Extracts from cells transfected with LexA-DBD cDNA can be used as a positive control LexA DNA-binding Domain antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with LexA DNA-binding Domain antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting