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Active Motif’s Targeted Next-Gen Bisulfite Sequencing Service offers significant advantages over the traditional Sanger-based method, most notably the ability to multiplex several amplicons from multiple bisulfite-converted DNA samples in a single Next-Gen sequencing reaction. Because 100X to 10,000X more data is generated for each cytosine, the resulting data is more statistically significant than Sanger sequencing. The service is an end-to-end assay and includes bisulfite conversion, primer design and testing, PCR amplification, library preparation, DNA sequencing and data analysis.
What our customers are saying about us:
"We used next-generation bisulfite sequencing and ChIP-Seq services to describe tumor suppressor function of KLF4 in pediatric leukemias. The quality of this service including the bioinformatic analysis was outstanding and essential for our project."
Daniel Lacorazza, PhD
Baylor College of Medicine
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Advantages of Targeted Next-Gen Bisulfite Sequencing
- More sequencing data results in greater statistical significance
- Use longer amplicons than Sanger Bisulfite Sequencing
- A more cost effective option for large-scale experiments using multiple cell types and/or amplicons
Figure 1: Heat map of Targeted Next-Gen Bisulfite Sequencing data.