LightSwitch™ Pathway Reporter Stable Cell Lines

measure pathway activation in response to treatment

LightSwitch Pathway Reporter Stable Cell Lines are the optimal solution for determining the response of specific pathways to treatment with various compounds or growth conditions in high-throughput. Each cell line contains a stably integrated human regulatory element, validated to be a biomarker of a specific biological pathway, driving the expression of the optimized RenSP luciferase gene.

LightSwitch Pathway Reporter Stable Cell Lines

Note: All Cell Lines MUST be used with the LightSwitch Luciferase Assay Reagents to obtain optimal results with our novel RenSP luciferase.

Please inquire in regards to our academic discount on the stable cell lines listed below. Active Motif also offers LightSwitch™ Validated Pathway Collections, which are sets of biomarkers validated to show significant activation or repression in response to various pathway-specific induction conditions.

 
Name Pathway Construct Format Cat No. Price  
Control 2 Reporter Cell Line (Hep G2) Housekeeping Control ACTB 1 vial 32232 $7,900 Buy
Control 2 Reporter Cell Line (HT1080) Housekeeping Control ACTB 1 vial 32202 $7,900 Buy
AhR v2 Reporter Cell Line (Hep G2) Dioxin/Tox CYP1A1 1 vial 32234 $7,900 Buy
BMP Reporter Cell Line (HT1080) Bone Morphogenesis BMP v2 SynthRE 1 vial 32206 $7,900 Buy
GR Reporter Cell Line (HT1080) Glucocorticoid Receptor GR SynthRE 1 vial 32210 $7,900 Buy
HSF Reporter Cell Line (HT1080) Heat Shock HSF SynthRE 1 vial 32213 $7,900 Buy
SREBP v1 Reporter Cell Line (Hep G2) Cholesterol LSS 1 vial 32251 $7,900 Buy
SREBP v1 Reporter Cell Line (HT1080) Cholesterol LSS 1 vial 32221 $7,900 Buy

LightSwitch Luciferase Pathway Reporter Stable Cell Line Overview

LightSwitch Pathway Reporter Stable Cell Lines simplifies the task of measuring the response of specific pathways to treatment with various compounds or growth conditions. In contrast to transiently transfected reporter vectors, the reporter lines stably express the RenSP reporter gene regulated by an element that is responsive to a specific pathway. Simply seed the cell line in multi-well plates, treat each well as desired, then perform high-throughput luciferase assays to measure the response of the regulatory element to the various treatments.

Key Applications

  • High-throughput Screening – LightSwitch Pathway Reporter cell lines are ideal for both primary and secondary high-throughput screening, and can be used in 96, 384 and 1536 well plates.
  • Extended Assay Length – Measure changes in expression over longer time courses than are possible with transient assays.
  • Pathway Profiling – Use a panel of reporter cell lines to characterize the effect of your compounds on a variety of biological pathways.
Dose response of AhR HepG2 and AhR HEK293 stable cell lines to increasing concentrations of omeprazole
Dose response of AhR HepG2 and AhR HEK293 stable cell lines to increasing concentrations of omeprazole.

The AhR HepG2 and AhR HEK293 stable cell lines were assayed for RenSP luciferase following treatment with omeprazole. The HEK293 cell line exhibits a weaker response to omeprazole due to HEK293 cells expressing lower levels of the aryl hydrocarbon receptor (AhR).

Custom Services are also available for creating a custom stable cell line of your choice.

LightSwitch Pathway Reporter Stable Cell Lines perform exceptionally in primary screens in 96-, 384- and 1536-well formats, and provide consistent, robust results.

Control 2 Reporter Cell Lines (HT1080 & Hep G2)

The LightSwitch™ Control 2 Reporter Cell Lines are used to normalize for non-specific effects when performing LightSwitch Luciferase Assays using our Pathway Reporter Stable Cell Lines. Each cell line contains a stably integrated LightSwitch ACTB Promoter Control construct (S717678), which is comprised of the promoter sequence of the ACTB (beta actin) gene cloned into the MCS of the LightSwitch Promoter Reporter Vector upstream of the RenSP luciferase gene.

The data at right shows the luciferase activity of both LightSwitch Control 2 Reporter Cell Lines (HT1080 & Hep G2) when assayed using the LightSwitch Luciferase Assay Kit without any induction. Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch Control 2 Reporter Cell Lines (HT1080 and Hep G2)
Click image to enlarge.

AhR v2 Reporter Cell Line (Hep G2)

The LightSwitch™ AhR v2 Reporter Cell Line (Hep G2) was designed for study of the aryl hydrocarbon receptor (AhR) and the regulation of biological responses to dioxins and toxicity. It contains a stably integrated LightSwitch Promoter Reporter GoClone® construct (S714555), which is comprised of the promoter sequence of the CYP1A1 (cytochrome P450, family 1, subfamily A, polypeptide 1) gene cloned into the MCS of the LightSwitch Promoter Reporter Vector upstream of the RenSP luciferase gene.

The data at right shows the luciferase activity of the LightSwitch AhR v2 Reporter Cell Line (Hep G2) when assayed using the LightSwitch Luciferase Assay Kit following induction with increasing amounts of Omeprazole (top) or TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) (bottom). Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch AhR v2 Reporter Cell Line (Hep G2) following induction with increasing amounts of Omeprazole
T-Test: 1.35E-05    Z′: 0.82
Click image to enlarge.


Graph showing the luciferase activity of the LightSwitch AhR v2 Reporter Cell Line (Hep G2) following induction with increasing amounts of Omeprazole
T-Test: 5.99E-07    Z′: 0.91
Click image to enlarge.

BMP Reporter Cell Line (HT1080)

The LightSwitch™ BMP Reporter Cell Line (HT1080) was designed for study of the Bone Morphogenesis pathway. It contains a stably integrated LightSwitch Synthetic Response Element reporter construct (S900030), which is comprised of repeats of a bone morphogenetic protein (BMP) binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the LightSwitch Long-range Enhancer Reporter Vector.

The data at right shows the luciferase activity of the LightSwitch BMP Reporter Cell Line (HT1080) when assayed using the LightSwitch Luciferase Assay Kit following induction with increasing amounts of bone morphogenetic protein 4 (BMP4). Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch BMP Reporter Cell Line (HT1080) following induction with increasing amounts of bone morphogenetic protein 4 (BMP4)
T-Test: 3.41E-05    Z′: 0.72
Click image to enlarge.

GR Reporter Cell Line (HT1080)

The LightSwitch™ GR Reporter Cell Line (HT1080) was designed for study of pathways regulated by the glucocorticoid receptor (GR). It contains a stably integrated LightSwitch Synthetic Response Element reporter construct (S900015), which is comprised of repeats of a glucocorticoid receptor binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the LightSwitch Long-range Enhancer Reporter Vector.

The data at right shows the luciferase activity of the LightSwitch GR Reporter Cell Line (HT1080) when assayed using the LightSwitch Luciferase Assay Kit following induction with increasing amounts of Dexamethasone (DMSO). Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch GR Reporter Cell Line (HT1080) following induction with increasing amounts of Dexamethasone (DMSO)
T-Test: 6.41E-07    Z′: 0.90
Click image to enlarge.

HSF Reporter Cell Line (HT1080)

The LightSwitch™ HSF Reporter Cell Line (HT1080) was designed for study of the Heat Shock pathway. It contains a stably integrated LightSwitch Synthetic Response Element reporter construct (S900017), which is comprised of repeats of a heat shock factor (HSF) binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the LightSwitch Long-range Enhancer Reporter Vector.

The data at right shows the luciferase activity of the LightSwitch HSF Reporter Cell Line (HT1080) when assayed using the LightSwitch Luciferase Assay Kit following induction by moving the plates to 43°C and then incubating at this elevated temperature for the times indicated (0-8 hours). Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch HSF Reporter Cell Line (HT1080) following induction by moving the plates to 43 degrees Centigrade and then incubating at this elevated temperature for the times indicated (0-8 hours)
T-Test: 1.42E-02    Z′: 0.60
Click image to enlarge.

SREBP v1 Reporter Cell Line (Hep G2)

The LightSwitch™ SREBP v1 Reporter Cell Line (Hep G2) was designed for study of the cholesterol biosynthesis pathway. It contains a stably integrated LightSwitch Promoter Reporter GoClone® construct (S711010), which is comprised of the promoter sequence of the LSS (lanosterol synthase or 2,3-oxidosqualene-lanosterol cyclase) gene cloned into the MCS of the LightSwitch Promoter Reporter Vector upstream of the RenSP luciferase gene. LSS catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D.

The data at right shows the luciferase activity of the LightSwitch SREBP v1 Reporter Cell Line (Hep G2) when assayed using the LightSwitch Luciferase Assay Kit following induction with increasing amounts of Lovastatin. Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch SREBP v1 Reporter Cell Line (Hep G2) following induction with increasing amounts of Lovastatin
T-Test: 7.05E-07    Z′: 0.89
Click image to enlarge.

SREBP v1 Reporter Cell Line (HT1080)

The LightSwitch™ SREBP v1 Reporter Cell Line (HT1080) was designed for study of the cholesterol biosynthesis pathway. It contains a stably integrated LightSwitch Promoter Reporter GoClone® construct (S711010), which is comprised of the promoter sequence of the LSS (lanosterol synthase or 2,3-oxidosqualene-lanosterol cyclase) gene cloned into the MCS of the LightSwitch Promoter Reporter Vector upstream of the RenSP luciferase gene. LSS catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D.

The data at right shows the luciferase activity of the LightSwitch SREBP v1 Reporter Cell Line (HT1080) when assayed using the LightSwitch Luciferase Assay Kit following induction with increasing amounts of Lovastatin. Please download the Data Sheet found under the Documents tab for more complete details.

Graph showing the luciferase activity of the LightSwitch SREBP v1 Reporter Cell Line (HT1080) following induction with increasing amounts of Lovastatin
T-Test: 6.24E-05    Z′: 0.66
Click image to enlarge.