Recombinant PRDM10 protein

Aliases: PFM7
Catalog No: 31396 Format: 20 µg $430 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant PRDM10 protein expressed in Sf9 cells and supplied in 25 mM HEPES, pH 7.5, 300 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

PRDM10 (PR Domain Containing 10) is a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. PRDM10 is a transcription factor that contains C2H2-type zinc-fingers and that possesses histone methyltransferase activity. PRDM10 also contains a positive regulatory domain which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant PRDM10 (accession number NP_004982.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 134 kDa (appears larger in SDS-PAGE gel image). The recombinant protein is >70% pure by SDS-PAGE.

 
PRDM10 Coomassie gel

Recombinant PRDM10 protein gel.
PRDM10 protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.