Recombinant PRDM14 protein

Aliases: PFM11
Catalog No: 31397 Format: 20 µg $430 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant PRDM14 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

PRDM14 (PR Domain Containing 14) is a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. PRDM14 possesses histone methyltransferase activity. PRDM14 is a transcription factor that has both positive and negative roles on transcription. It is required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. PRDM14 may play an essential role in germ cell development at two levels: the reacquisition of potential pluripotency, including Sox2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. PRDM14 directly up-regulates the expression of pluripotency gene Oct4 through its proximal enhancer. PRDM14 binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3.'

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant PRDM14 was expressed in a baculovirus expression system as the full length protein (accession number NP_004982.2) with an N-terminal FLAG-Tag. The molecular weight of PRDM14 is 67 kDa. The purity of the protein is > 80% by SDS-PAGE.

 
Recombinant PRDM14 protein gel.

Recombinant PRDM14 protein gel.
PRDM14 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.