Recombinant PRMT7 protein

Catalog No: 31395 Format: 20 µg $430 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant PRMT7 protein expressed in Sf9 cells and supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100 and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

Background

PRMT7 (Protein Arginine Methyltransferase 7) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT7 specifically mediates the symmetrical dimethylation of histone H4 at Arg3 to form H4R3me2s. PRMT7 plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruitment of DNA methyltransferases at these sites. PRMT7 may also play a role in embryonic stem cell (ESC) pluripotency. PRMT7 is also able to mediate arginine methylation of histone H2A and myelin basic protein (MBP) in vitro. However, the biological relevance of such results is unclear.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant PRMT7 (accession number NP_061896.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 80.1 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
PRMT7 Coomassie gel

Recombinant PRMT7 protein gel.
PRMT7 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.