100 µg HeLa acid extract at 0.5 µg/µl.
HeLa acid extract was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line. Acid extraction is performed under conditions that are optimized for solubility of histone proteins.
The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.
Acid extracts are commonly utilized for isolation and purification of histones. HeLa acid extract is recommended for use in various applications across many research areas, including cancer, virology, and gene mapping studies.
Human Cervix Adenocarcinoma
HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF).
Acid extracts have been quality control tested by Western blot.
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.