L3MBTL1 antibody (pAb)

Aliases: L3MBTL, ZC2HC3, H-L(3)MBT, dJ138B7.3
Catalog No: 39182 Format: 100 µl $435 Buy
Catalog No: 39183 Format: 10 µl $105 Buy

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Antibody Type:
Molecular Weight:
100 kDa
Human, Other (Wide Range)


ChIP Validated Western Blot Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This L3MBTL1 antibody was raised against a His-Tagged fusion protein corresponding to amino acids 1-215 of human L3MBTL1.


Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


L3MBTL1 antibody (pAb) tested by Western blot.

L3MBTL1 antibody tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with L3MBTL1 antibody (1:2,000 dilution).


L3MBTL1 is a human homolog of the Drosophila l(3)mbt protein. Located on chromosome 20q12, L3MBT1 is a member of the malignant brain tumor (MBT) protein family and directly compacts chromatin in a strictly histone methylation dependent fashion. L3MBTL1 functions as a transcriptional repressor in a manner that requires its three MBT domains, which were predicted to bind methylated lysines of histones. L3MBTL1 binds to Lys20 of Histone H4 that has been mono- or dimethylated, as well as to Histone H1 isotype b methylated at lysine 26. L3MBTL1 is a complex molecule with a Zinc-finger and a sterile alpha motif (SAM).

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for L3MBTL1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with L3MBTL1 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting