L3MBTL2 antibody (pAb)

Aliases: L3MBT, H-l(3)mbt-l
Catalog No: 39569 Format: 100 µl $435 Buy
Catalog No: 39570 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
90 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Immunogen

This L3MBTL2 antibody was raised against a His-Tagged fusion protein containing amino acids 1-230 of human L3MBTL2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
L3MBTL2 antibody (pAb) tested by immunoprecipitation.

L3MBTL2 antibody (pAb) tested by Immunoprecipitation.
10 µl of L3MBTL2 antibody was used to immunoprecipitate L3MBTL2 from 500 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the L3MBTL2 antibody at a dilution of 1:2,000.

L3MBTL2 antibody (pAb) tested by Western blot.

L3MBTL2 pAb tested by Western blot.
Detection of L3MBTL2 by Western blot analysis with 20 µg HeLa nuclear extract and a 1:2,000 dilution of L3MBTL2 pAb.

Background

L3MBTL2 (Lethal (3) malignant brain tumor-like 2 protein) is a putative member of the polycomb group family and is the human homolog of Drosophila Sfmbt, critical for Hox gene silencing. L3MBTL2 contains one FCS-type zinc finger and four MBT (malignant brain tumor) repeats. L3MBTL2 associates with chromatin-remodeling complexes and helps inhibit the expression of proteins that trigger the cells into mitosis. L3MBTL2 is a part of a complex involved in the G0 phase of the cell cycle. This complex contains other proteins such as E2F-6, Max or HP1 gamma, and participates in transcriptional repression.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for L3MBTL2 pAb.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting