Lamin A/C antibody (mAb)

RRID: AB_2793218
Clone: 3A6-4C11
Aliases: LMNA, FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B
Catalog No: 39287 Format: 100 µl $455 Buy
Catalog No: 39288 Format: 10 µl $110 Buy
Catalog No: 39087 Format: 50 µl $265 Buy

Request a quote for a bulk order Request Quote


Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Culture Supernatant
Host:
Mouse
Molecular Weight:
75 kDa
Reactivity:
Hamster, Human, Monkey, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:4000 dilution
ICC/IF: 1:500 - 1:2000 dilution

Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation.

This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91189.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq
IP
WB
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

Buffer

Cell culture supernatant containing mouse IgG, 30% glycerol, 0.035% sodium azide.

References

 

Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence.
Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11). Cells were fixed with 3.7% PFA for 15 minutes at room temperature, permeabilized with PBS + 0.5% Triton™ X-100 and stained with Lamin A/C mAb (2 µg/ml dilution) followed by 1:500 dilution Chromeo™ 546 Goat anti-mouse IgG (Cat No. 15033) (top panel) and DAPI (bottom panel).

Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot.
Whole-cell extract (20 µg) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 0.5 µg/ml.

Background

Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Lamin A/C antibody (Clone 3A6-4C11).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Lamin A/C antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting