RING1B antibody (mAb)

RRID: AB_2615006
Clone: 3-3
Aliases: ring finger protein 2, RNF2, BAP1, DING, BAP-1, HIPI3, RING2
Catalog No: 39663 Format: 100 µg $455 Buy
Catalog No: 39664 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
40 kDa
Reactivity:
Human, Monkey, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ChIP-Seq: 4 µl each

Published Applications:
ChIP-Seq
ChIP
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This Ring1B antibody was raised against the recombinant full-length mouse protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Ring1B antibody (mAb) tested by ChIP-chip.

Ring1B antibody (mAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human embryonic stem cells and 4 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.

Ring1B antibody (mAb) tested by ChIP-Seq.

Ring1B antibody (mAb) tested by ChIP-Seq.
ChIP was performed using Ring1B antibody with 30 µg chromatin from the wild-type mouse ESC and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.

Background

Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

Learn more about the PRC1 Complex and Stem Cell Identity

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RING1B antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use RING1B antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting