HP1α antibody (pAb)

Aliases: CBX5, HP1, Hp1a
Catalog No: 39295 Format: 200 µl $435 Buy
Catalog No: 39296 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
24 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Immunogen

This HP1 alpha antibody was raised against a recombinant protein corresponding to full-length recombinant mouse HP1 alpha.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
HP1α antibody (pAb) tested by Western blot.

HP1 alpha pAb tested by Western blot.
Detection of HP1 alpha by Western blot analysis. Nuclear extract of HeLa cells (20 µg/lane) stained with HP1 alpha pAb at a 1:1,000 dilution.

Background

HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (α, β and γ) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1α is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1α plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HP1 alpha antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting