HP1 gamma phospho Ser93 antibody (pAb)

RRID: AB_2793292
Aliases: HECH, CBX3, HP1Hs-gamma
Catalog No: 39655 Format: 100 µl $460 Buy
Catalog No: 39656 Format: 10 µl $115 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
25 kDa


Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution


This HP1 gamma phospho Ser93 antibody was raised against a peptide containing phospho Ser93 of human HP1 gamma.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


HP1 gamma phospho Ser93 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with HP1 gamma phospho Ser93 pAb (1:500 dilution). Lane 1: no treatment. Lane 2: cells treated with colcemid.

HP1 gamma phospho Ser93 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: unmodified HP1 gamma peptide.
Lane 2: phospho Ser93 HP1 gamma peptide.
Lane 3: phospho Ser92 HP1 alpha peptide.
Lane 4: phospho Ser89 HP1 beta peptide.


Heterochromatin Protein 1 gamma (HP1 gamma) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 gamma contains a Chromo domain and a Chromo shadow domain. Through the Chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Phosphorylation of serine 93 of HP1 gamma increases during mitosis.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HP1 gamma phospho Ser93 pAb.


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.


This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting