AML-2/Runx3 antibody (pAb)
|Aliases: CBFA3, PEBP2aC|
|Catalog No: 39301||Format: 100 µl||$455||Buy|
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Applications Validated by Active Motif:
EMSA: 4 - 6 µl per assay
The addition of Tris-glycine electrophoresis buffer for supershift assays is recommended.
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
This AML-2 antibody was raised against a full-length protein corresponding to human AML-2/Runx3.
PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.
AML-2 (acute myeloid leukemia 2 protein) also known as Runx3 (Runt-related transcription factor 3) is a member of the DNA binding alpha subunit of the heterodimeric transcription factor complex PEBP2/CBF. AML-2 can either activate or repress transcription, and it also interacts with other transcription factors. AML-2 expression is regulated by AML-1/Runx1.
Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for AML-2/Runx3 antibody.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AML-2/Runx3 antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting