AML-2/Runx3 antibody (pAb)

RRID: AB_2793222
Aliases: CBFA3, PEBP2aC
Catalog No: 39301 Format: 100 µl $455 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
44 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
EMSA: 4 - 6 µl per assay

The addition of Tris-glycine electrophoresis buffer for supershift assays is recommended.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP, ChIP-qPCR
WB
ICC
IHC(P)
EMSA

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This AML-2 antibody was raised against a full-length protein corresponding to human AML-2/Runx3.

Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

References

 

AML-2/Runx3 antibody tested by supershift.
Detection of AML-2/Runx3 by EMSA analysis. AML-2/Runx3 antibody was used in a supershift assay (EMSA) with Raji nuclear extract (lane 4).

Background

AML-2 (acute myeloid leukemia 2 protein) also known as Runx3 (Runt-related transcription factor 3) is a member of the DNA binding alpha subunit of the heterodimeric transcription factor complex PEBP2/CBF. AML-2 can either activate or repress transcription, and it also interacts with other transcription factors. AML-2 expression is regulated by AML-1/Runx1.

Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for AML-2/Runx3 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AML-2/Runx3 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting