Raji nuclear extract

Catalog No: 36023 Format: 200 µg $175 Buy

Contents

2 x 100 µg of Raji nuclear extract at 2.5 µg/µl.

Background

Raji nuclear extract was prepared from cell cultures of the Raji Burkitt's Lymphoma cell line. This lymphoblastoid cell line was derived from a Nigerian patient with Burkitt's lymphoma and is the first continuous cell line of hematopoietic origin. These cells are most commonly used in studies related to hematology and related cancers, such as leukemia and lymphoma. Raji cells are a line of Epstein-Barr virus (EBV)-transformed lymphocytes and thus harbor latent EBV genomes and various chromosomal translocations, making them an ideal source for material for cytogenetic analysis. In addition, these cells express numerous surface Fc receptors and are routinely used for detection and analysis of immune complexes.

Application Notes

Raji nuclear extract is specifically recommended for studies related to 1) hematology, 2) leukemia and lymphoma, 3) cytogenetics, and 4) immunology.

Extract Origin

Human B Lymphoblast (Burkitt's)

Extract Composition

Raji nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

 
Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.

Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.
Different amounts of nuclear extracts from untreated Raji (purple bars) and HeLa (copper bars) cells are tested for FKHR activity using the TransAM FKHR Kit.

Figure 1: NFκB family profiling of DNA binding activation in various cell lines.

Figure 1: NFκB family profiling of DNA binding activation in various cell lines.
Nuclear extracts prepared from HeLa, HeLa treated with TNF-α, Jurkat, Jurkat treated with PMA and calcium ionophore, and Raji cells were assayed at 10 µg/well for p65, p50, c-Rel, p52 and RelB activity using the TransAM NFκB Family Kit.

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.