Nucleolin antibody (mAb)

Clone: 3G4B20
Aliases: NCL, C23
Catalog No: 39541 Format: 200 µl $435 Buy
Catalog No: 39542 Format: 10 µl $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Culture Supernatant
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Human, Mouse, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:500 dilution
WB: 1:500 - 1:2,000 dilution

Also available as an AbFlex® engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91231.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.

Buffer

Concentrated culture supernatant.

References

 
Nucleolin antibody (mAb) tested by immunofluorescence.

Nucleolin mAb (Clone 3G4B20) tested by immunofluorescence.
Staining of HeLa cells with Nucleolin mAb (Clone 3G4B20) (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Nucleolin antibody (mAb) tested by Western blot.

Nucleolin mAb (Clone 3G4B20) tested by Western blot.
HeLa whole-cell extract (15 µg/lane) probed with Nucleolin mAb (Clone 3G4B20) (1:2,000 dilution).

Background

Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Nucleolin antibody (Clone 3G4B20).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Nucleolin antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting