Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

AbFlex® Nucleolin antibody (rAb)

Catalog No: 91231 Format: 100 µg $375 Buy Now
Catalog No: 91232 Format: 10 µg $75 Buy Now

Request a quote for a bulk orderBulk Order Button

Antibody Type:Recombinant
Purification:Protein A Chromatography
Molecular Weight:100 kDa
Reactivity:Canine, Human


Western Blot Validated

for AbFlex Nucleolin antibody (rAb)Application Notes

Validated Applications:
WB: 0.1 - 1 µg/mL

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39541.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AbFlex Nucleolin antibody (rAb)Immunogen

This antibody was raised against a salt-extracted nuclear matrix preparation from MDCK (canine kidney) cells.

for AbFlex Nucleolin antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex Nucleolin antibody (rAb)References

AbFlex<sup>®</sup> Nucleolin antibody (rAb) tested by Western blot.

AbFlex® Nucleolin antibody (rAb) tested by Western Blot.
25 µg of HeLa cell nuclear extract (Lane 1) or 30 µg K562 cell nuclear extract (Lane 2) was run on SDS-PAGE and probed with AbFlex Nucleolin antibody at 0.5 µg/ml.

for AbFlex Nucleolin antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), AbFlex® Nucleolin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

for AbFlex Nucleolin antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Nucleolin antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® Nucleolin antibody (rAb)

Data Thumbnails

AbFlex<sup>®</sup> Nucleolin antibody (rAb) tested by Western blot.

Western blot of AbFlex® Nucleolin antibody (rAb).
(Click image to enlarge and see details.)