Ubn1 antibody (pAb)

Aliases: VT, VT4
Catalog No: 39869 Format: 100 µl $435 Buy
Catalog No: 39870 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
145 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This Ubinuclein antibody was raised against a recombinant protein corresponding to amino acids 1-345 of human Ubn1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Ubn1 antibody (pAb) tested by Western blot.

Ubn1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 μg) probed with Ubn1 antibody at a dilution of 1:500.

Background

UBN1 (Ubinuclein 1, also known as HIRA binding protein) is a ubiquitously expressed nuclear protein that acts as a novel regulator of senescence. UBN1 is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. It is expressed in a number of cancer cell lines and tumors and may be required for replication-independent chromatin assembly. UBN1 is part of a large multi-subunit protein complex that interacts with CEBPA and Epstein-Barr virus BLZF1. It co-localizes with HIRA, PML and SP100 in the PML bodies of senescent cells. When overexpressed, UBN1 accumulates in the nucleus of cells defective for cytokinesis.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Ubn1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting