HIRA antibody (mAb)

Clone: WC119.2H11
Aliases: TUP1, DGCR1, TUPLE1
Catalog No: 39557 Format: 100 µg $435 Buy
Catalog No: 39558 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
120 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 1 - 5 µg/ml dilution

Published Applications:
ICC/IF
See reference for experimental details. Individual optimization may be required.

Immunogen

This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
HIRA antibody (mAb) tested by Western blot.

HIRA mAb (Clone WC119.2H11) tested by Western blot.
5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml.

Background

HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1α is involved in the formation of the senescence-associated heterochromatin foci.

Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for HIRA antibody (Clone WC119.2H11).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HIRA antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting